PDF《鳖甲药材指纹图谱与其抗肝纤维化作用的谱效关系研究=》

・ORMS・ 中草药 Chinese qraditional and eerbal arugs 第QR 卷第1T 期OM1Q 年V月= = = = = 鳖甲药材指纹图谱与其抗肝纤维化作用的谱效关系研究= 肖云芝 NI=O ,于= = 超N,刘朝勇 NI=O ,万= = 露N,徐平华 N ,韩= = 晋NG ,袁海龙 NG = NK= 中国人民解放军第三二医院,北京= = NMMMPV= OK= 成都中医药大学药学院,四川= 成都= = SNNNPT= 摘= = 要:目的= = 研究不同产地鳖甲药材的 emiC 指纹图谱与其抗纤维化作用的谱效关系,揭示鳖甲药材抗肝纤维化作用的 药效成分组 .

方法= = emiCJaAa 法测定了 NO 个产地鳖甲药材样品,采用中药色谱指纹图谱相似度评价系统 OMMQ= A 进行 评价,建立了共有模式,并进行主成分分析(mCA) .以iuJO 肝星状细胞为模型研究其对肝细胞的抑制效果,采用 pmpp 软 件对数据进行相关分析,研究谱效相关性.结果= = NO 个产地鳖甲药材 emiC 指纹图谱中共有 T 个特征共有峰,其中峰 O、R 与iuJO 肝星状细胞抑制效果(A 值)的相关性呈现中等程度相关,峰Q的相关性最强.由mCA 可知,峰Q的量对鳖甲质量 有着重要的影响.结论= = NO 个产地鳖甲药材对 iuJO 肝星状细胞均有抑制作用,emiC 指纹图谱与其抗纤维化作用之间有一 定的相关性,为进一步控制鳖甲药材内在质量提供参考.= 关键词:鳖甲;

指纹图谱;

抗肝纤维化作用;

主成分分析;

谱效关系;

iuJO 肝星状细胞= 中图分类号:oOUSKMO=文献标志码:A=文章编号:MORP=J=OSTMEOMNQFNT=J=ORMS=J=MR= alfW=NMKTRMNLjKissnKMORPJOSTMKOMNQKNTKMNR= ppectrum-activity=relationship=on=anti-hepatic=fibrosis=efficacy=of=Trionycis Carapax ufAl=vunJzhiNI=O I=vr=ChaoN I=ifr=ChaoJyongNI=O I=tAk=iuN I=ur=mingJhuaN I=eAk=ginN I=vrAk=eaiJlongN = NK=PMO=jilitary=eospital=of=Chinese=meople'

s=iiberation=ArmyI=Beijing=NMMMPVI=China= OK=College=of=mharmacyI=Chengdu=rniversity=of=qraditional=Chinese=jedicineI=Chengdu=SNNNPTI=China= AbstractW=lbjective= = qo=investigate=the=spectrumJactivity=relationship=between=emiC=of=Trionycis=Carapax=from= different= regions= and=its= antiJhepatic=fibrosis=efficacyI=in=order=to=reveal=the=±active=component=group =for=antiJhepatic=fibrosis=efficacy=of=Trionycis= CarapaxK= jethods= = qhe= samples= from= NO= regions= were= determined= with= the= emiCJaAa= methodK= qhe= representative= standard= fingerprint=was=calculated=using=the=pimilarity bvaluation pystem for Chromatographic cingerprint of Traditional Chinese jedicine Esersion=OMMQAFK=qhe=common=patten=and=the=principal=component=analysis=EmCAF=were=establishedK=rsing=the=iuJO=hepatic=stellate= cell= as= a= hepatic= fibrosis= model= of= Trionycis Carapax= to= study= its= inhibition= on= the= hepatic fibrosis= cellsK= qhe= spectrumJeffect= relationship=was=studied=by=pmpp=NVKM=softwareK=oesults= =peven=common=peaks=in=the=emiCJfingerprint=of=Trionycis Carapax=were= obtainedK=ft=was=tentatively=concluded=that=peaks=O=and=R=related=better=to=the=inhibitory=effect=of=iuJO=cells=Ela=valueF=in=the=seven= characteristic=peaksK=meak=Q=had=the=strongest=correlationK=And=the=quality=of=Trionycis Carapax=was=influenced=by=the=concentration=of= peak=Q=obtained=by=mCAK=Conclusion== All=the= samples=could=inhibit=the= proliferation=of= iuJO=hepatic=stellate=cell=in=some=extentK= qhere=may=be=a=certain=relationship=between=emiC=fingerprint=and=antiJhepatic=fibrosis=efficacyK=fn=additionI=the=research=could=be=used= as=the=quality=control=method=of=Trionycis CarapaxK= hey= wordsW= Trionycis CarapaxX= fingerprintX= antiJhepatic= fibrosis= efficacyX= principal= component= analysisX= spectrumJactivity= relationshipX=iuJO=hepatic=stellate=cell= = 鳖甲 Trionycis Carapax 为鳖科动物鳖 Trionyx= sinensis= tiegmann 的背甲xNz ,具有滋阴潜阳、退热 除蒸、软坚散结的功效.相关药理研究表明,鳖甲 提取物能促进早期或者晚期肝硬化肝细胞恢复及纤 维组织重吸收xOJPz .= 中药的 多成分、多靶点、多渠道 特点表明= = 收稿日期:OMNQJMPJNV= 基金项目:国家新药创制重大专项(OMNNwuMVOMNJOMNJNQ) ;