PDF《及其相关抗性物质》

植物保护学报 Journal of Plant Protection, 2019,

46 (2) : 425-433 DOI: 10.

13802/j.cnki.zwbhxb.2019.2018050 基于EPG技术分析杭菊两主栽品种对三种菊蚜抗性 及其相关抗性物质 祝愿1 王梦馨1 崔林1 沈子尧2 周建松3 韩宝瑜1* (1. 中国计量大学浙江省生物计量及检验检疫技术重点实验室, 杭州 310018;

2. 桐乡市农机推广站, 浙江 桐乡 314500;

3. 桐乡市农业技术推广服务中心, 浙江 桐乡 314500) 摘要:为比较杭白菊主栽品种早、 晚小洋菊对菊小长管蚜 Macrosiphoniella sanborni、 棉蚜 Aphis gossypii、 桃蚜Myzus persicae的抗性, 通过刺吸电位技术 (electrical penetration graph, EPG) 研究菊蚜 在菊顶叶上刺吸行为, 并检测了菊顶叶主要化合物.结果表明: 在晚小洋菊上, 菊小长管蚜的E

1、 E2波平均持续时间4.

31、 3.47 min, 分别短于在早小洋菊上的4.

63、 3.75 min;

棉蚜、 桃蚜的E

1、 E2波 平均持续时间分别为 4.

32、 4.72 min 和4.

92、 4.64 min;

3 种蚜虫的平均刺探次数均大于在早小洋菊 上的平均刺探次数.聚类分析结果显示, 2个主栽品种之间抗性存在差异.早小洋菊顶叶平均可 溶性糖和可溶性蛋白含量分别为2.71 mg/g和25.36 mg/g, 均高于晚小洋菊;

早小洋菊顶叶总酚含量 为0.24 mg/g, 显著低于晚小洋菊;

早、 晚小洋菊总黄酮含量分别为3.46 mg/g和3.37 mg/g.早小洋 菊顶叶可溶性糖、 可溶性蛋白含量基本上与每种菊蚜的E

1、 E2波持续时间显著正相关, 总酚含量、 总黄酮含量与每种菊蚜的E1或E2波持续时间显著负相关.推测早小洋菊对于菊长管蚜的抗性稍 弱于晚小洋菊, 2种杭白菊对棉蚜或桃蚜的抗性相当, 且菊叶中的这4种物质含量与抗蚜性相关. 关键词: 杭白菊;

菊蚜;

抗蚜性;

刺吸电位技术;

抗性物质 Analysis of resistance of two elite Chrysanthemum morifolium cultivars to three species of aphids using EPG techniques and their potential resistant substances Zhu Yuan1 Wang Mengxin1 Cui Lin1 Shen Ziyao2 Zhou Jiansong3 Han Baoyu1* (1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection &

Quarantine, China Jiliang University, Hangzhou 310018, Zhejiang Province, China;

2. Extension Station for Agricultural Machinery of Tongxiang City, Tongxiang 314500, Zhejiang Province, China;

3. Extension Center for Agricultural Techniques of Tongxiang City, Tongxiang 314500, Zhejiang Province, China) Abstract: In order to compare the differences in the resistance to three species of aphids (Macrosiphoni- ella sanborni, Aphis gossypii and Myzus persicae) between two elite Hangbaiju (Chrysanthemum mori- folium) cultivars: Zaoxiaoyangju and Wanxiaoyangju, the probing behaviors of these aphids on the api- cal leaves of the chrysanthemum plants were studied by using electrical penetration graph (EPG) tech- niques, and the main compounds of these apical leaves were also analyzed. The results showed that the durations of E1 and E2 waves in M. sanborni on the cultivar Wanxiaoyangju (4.31 min and 3.47 min, re- spectively) were shorter than those on the cultivar Zaoxiaoyangju (4.63 min and 3.75 min);

the dura- tions of E1 and E2 waves in A. gossypii and M. persicae were 4.32, 4.72 min and 4.92, 4.64 min, respec- tively. The average numbers of probes of the three aphids on Wanxiaoyangju were higher than those on 基金项目: 浙江省自然科学基金项目 (LY17C140002) , 浙江省大学生科技创新项目 (2017R409055) * 通信作者(Author for correspondence) , E-mail: [email protected] 收稿日期: 2018-02-18